ecl plus hypersensitive luminescence solution pe0010 Search Results


phthalates polyethylene standard  (ZeptoMetrix corporation)
N/A
Consumer Safety Standards Phthalates in Polyethylene QC Standards Polyethylene (PE) is one of the world’s most common plastics. PE is used in a variety of common consumer products including children’s toys and care items. Current
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ecl substrate reagent  (Solarbio Inc)
90
Solarbio Inc ecl substrate reagent
Ecl Substrate Reagent, supplied by Solarbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plus+hypersensitive+luminescence+solution+pe0010/ecl+reagent/pmc07373746-96-6-10
Average 90 stars, based on 1 article reviews
ecl substrate reagent - by Bioz Stars, 2026-09
90/100 stars
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146nd cd8a  (fluidigm)
93
fluidigm 146nd cd8a
LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), <t>CD8</t> + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
146nd Cd8a, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plus+hypersensitive+luminescence+solution+pe0010/Anti-PE+(PE001)-165Ho/bio_rxiv__531236-115-33-34
Average 93 stars, based on 1 article reviews
146nd cd8a - by Bioz Stars, 2026-09
93/100 stars
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3156005b cd74 ln2 fluidigm er  (fluidigm)
93
fluidigm 3156005b cd74 ln2 fluidigm er
LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), <t>CD8</t> + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
3156005b Cd74 Ln2 Fluidigm Er, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plus+hypersensitive+luminescence+solution+pe0010/Anti-PE+(PE001)-156Gd/ppr0653532-314-148-151
Average 93 stars, based on 1 article reviews
3156005b cd74 ln2 fluidigm er - by Bioz Stars, 2026-09
93/100 stars
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145nd-siglecf-pe/anti-pe, e50-2440/fluidigm, pe001  (Becton Dickinson)
90
Becton Dickinson 145nd-siglecf-pe/anti-pe, e50-2440/fluidigm, pe001
LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), <t>CD8</t> + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
145nd Siglecf Pe/Anti Pe, E50 2440/Fluidigm, Pe001, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plus+hypersensitive+luminescence+solution+pe0010/pe+anti+mouse+cd170++siglecf++e50+2440+/pmc06537751-298-35-31
Average 90 stars, based on 1 article reviews
145nd-siglecf-pe/anti-pe, e50-2440/fluidigm, pe001 - by Bioz Stars, 2026-09
90/100 stars
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sumo protease  (Genecopoeia)
93
Genecopoeia sumo protease
LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), <t>CD8</t> + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).
Sumo Protease, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plus+hypersensitive+luminescence+solution+pe0010/CoolCutter+SUMO+Protease/10__1155_slash_2015_slash_378786-37-9-11
Average 93 stars, based on 1 article reviews
sumo protease - by Bioz Stars, 2026-09
93/100 stars
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3145006b  (fluidigm)
92
fluidigm 3145006b

3145006b, supplied by fluidigm, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plus+hypersensitive+luminescence+solution+pe0010/Anti-PE+(PE001)-145Nd/pmc08443274-10-7-5
Average 92 stars, based on 1 article reviews
3145006b - by Bioz Stars, 2026-09
92/100 stars
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protein extraction kit  (Signalway Antibody)
86
Signalway Antibody protein extraction kit

Protein Extraction Kit, supplied by Signalway Antibody, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plus+hypersensitive+luminescence+solution+pe0010/anti+extraction+kit+protein/pmc12335446-280-12-16
Average 86 stars, based on 1 article reviews
protein extraction kit - by Bioz Stars, 2026-09
86/100 stars
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sds protein loading buffer pe0020  (Servicebio Inc)
86
Servicebio Inc sds protein loading buffer pe0020

Sds Protein Loading Buffer Pe0020, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+plus+hypersensitive+luminescence+solution+pe0010/buffer+loading/pm41898518-200-16-73
Average 86 stars, based on 1 article reviews
sds protein loading buffer pe0020 - by Bioz Stars, 2026-09
86/100 stars
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biotin anti-phycoerythrin (pe)  (BioLegend)
N/A
Biotin anti-phycoerythrin (PE) [PE001]; Isotype: Mouse IgG1, κ; Reactivity: 2nd Step; Apps: FC; Size: 25 μg
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purified anti-phycoerythrin (pe) (maxpar ready)  (BioLegend)
N/A
Purified anti-phycoerythrin (PE) (MaxPar Ready) [PE001]; Isotype: Mouse IgG1, κ; Reactivity: 2nd Step; Apps: FC, CyTOF; Size: 100 μg
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econazole nitrate crs  (European Directorate for the Quality of Medicines and HealthCare)
N/A
Econazole nitrate CRS
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Image Search Results


LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), CD8 + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

Journal: bioRxiv

Article Title: Tumor-Intrinsic Response to IFNγ Shapes the Tumor Microenvironment and Anti-PD-1 Response in NSCLC

doi: 10.1101/531236

Figure Lengend Snippet: LLC-NT or LLC-sh21 ( Socs1 knockdown) cells were injected into the left lung lobes of mice. After 2 weeks, mice were sacrificed and their tumor-bearing lung lobes were isolated for either flow cytometry or FFPE and T cell staining by immunofluorescence. Representative images of T cell staining from (A) A LLC-NT tumor or (B) A LLC-sh21 tumor at 40X magnification (Scale Bar 100µm) showing CD3 + cells (green), CD8 + cells, (red) and DAPI (blue). Quantification of (C) CD3 + T cells and (D) CD8 + T cells per high power field (HPF) in LLC-NT versus LLC-sh21 tumors (6 tumors in each group). T cell numbers per HPF were averaged over 4 experiments (6 random fields per tumor × 4 staining experiments=24 fields averaged/tumor in total). (E-H) Flow cytometry analysis of T cells was performed using 3 experimental replicates × 3 tumor bearing lung lobes each, for a total of 9 lung lobes per the experimental conditions of “LLC-NT” or “LLC-sh21”. (E) the percentage of PD-1 expressing CD8 + T cells, (F) double-positive PD-1/CD69 expressing CD8 + T cells gated as a percentage of all CD8 + T cells. (G-H) Single cell suspensions were either unstimulated (treated with Brefeldin and Monensin alone) or stimulated (treated with Brefeldin, Monensin, and PMA/Ionomycin) for 5 hours and analyzed by flow cytometry for (G) the percentage of single positive IFNγ expressing CD8 + T cells or (H) double-positive IFNγ/TNFα expressing CD8 + T cells gated as a percentage of all CD8 + T cells. Empty circles represent the LLC-NT condition while the solid black squares represent the LLC-sh21 condition. Error bars represent the mean of the data ±SEM after a student’s t-test (6C-F) or a two-way ANOVA (6G-H) (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

Article Snippet: Antibodies and Reagents: 89Y-CD45, Fluidigm, Clone 30-F11; 141Pr-Gr1 (Ly6C/Ly6G), Fluidigm, Clone RB6-8C5; 142Nd-CD11c, Fluidigm, Clone N418; 143Nd-GITR, Fluidigm, Clone DTA1; 144Nd-MHC class I, Fluidigm, Clone 28-14-8; 145Nd-SiglecF-PE/anti-PE, BD, Clone E50-2440/ Fluidigm, Clone PE001; 146Nd-CD8a, Fluidigm, Clone 53-6.7; 147Nd-p-H2AX[Ser139], Fluidigm, Clone JBW301; 148Nd-CD11b, Fluidigm, Clone M1/70; 149Sm-CD19, Fluidigm, Clone 6D5; 150Nd-CD25, Fluidigm, Clone3C7; 151Eu-CD64, Fluidigm, Clone X54-5/7.1; 152Sm-CD3e, Fluidigm, Clone 145-2C11; 153Eu-PD-L1, Fluidigm, Clone 10F.9G2; 154Sm-CTLA4, Fluidigm, Clone UC10-4B9; 155Gd-IRF4, Fluidigm, Clone 3E4; 156Gd-CD90.2(Thy-1.2), Fluidigm, Clone 30-H12; 158Gd-FoxP3, Fluidigm, Clone FJK-16s; 159Tb-PD-1, Fluidigm, Clone RMP1-30; 160Gd-CD80/86-FITC/anti-FITC, BD, Clone 16-10A1/BD Clone BL1/Fluidigm, Clone FIT22; 161Dy-INOS, Fluidigm, Clone 4B10; 162Dy-Tim3, Fluidigm, Clone RMT3-23; 163Dy-CXCR3-APC/anti-APCCXCR3-173, Biologend/Fluidigm, Clone APC003; 164Dy-IkBa, Fluidigm, Clone L35A5;165Ho-Beta-catenin (active), Fluidigm, Clone D13A1; 166Er-Arg1, Fluidigm, Clone 6D5; 167Er-NKp46, 2 Fluidigm, Clone 9A1.4; 168Er-Ki-67; Fluidigm, Clone Ki-67; 169Tm-Ly-6A/E (Sca-1), Fluidigm, Clone D7; 170Er-CD103-Biotin/anti-Biotin, Biolegend, Clone 2E7/Fluidigm, Clone 1D4-C5; 171Yb-CD44, Fluidigm, Clone IM7; 172Yb-CD4, Fluidigm, Clone RM4-5; 173Yb-CD117 (ckit), Fluidigm, Clone 2B8; 174Yb-Lag3, Fluidigm, Clone M5/114.15.2;175Lu-CD127, Fluidigm, Clone A7R34; 176Yb-ICOS, Fluidigm, Clone 7E.17G9; 191Ir, 193Ir-Intercalator, Cell-ID; 195Pt Cisplatin 5 uM, Cell-ID; 140Ce, 151Eu, 153 Eu, 165Ho, 175Lu Normalization Beads; Cell ID 20-Plex Pd Barcoding Kit, Fluidigm,#201060 (102, 104, 105, 106, 108, 110 Pd Bar Codes); Benzonase, Sigma #E1014-5KU(1:10,000) in HBSS.

Techniques: Injection, Isolation, Flow Cytometry, Staining, Immunofluorescence, Expressing

Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-PE 145Nd clone PE001 , Fluidigm , 3145006B.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry